Overcoming dormancy and different temperatures in dioclea. Crystal structure of dioclea violacea lectin and a comparative study. See status, confidence level, source for definitions. This page was last edited on 18 january 2015, at 15. Less potency and efficacy was observed forcanavalia maritima, dioclea guianensis, anddioclea violacea while very low. Dioclea multiflora boykins clusterpea discover life.
These findings were confirmed by mass spectrometry analysis proving the existence of just one lectin, composed of three chains. Dwl was purified by affinity chromatography on a sephadex g50 column and ion exchange chromatography on a hitrap sp xl column. An dioclea violacea in uska species han magnoliopsida nga ginhulagway ni george bentham. Pdf mass spectrometry and xray diffraction analysis of two. Crystallization and characterization of an inflammatory lectin purified from the seeds of dioclea wilsonii article pdf available in molecules 166. The antitumor activity of dvl, a lectin purified from dioclea violacea seeds, on the u87 human glioma cell line was evaluated and compared with canavalia ensiformis lectin cona. Dioclea violacea lectin dvl is a protein that recognizes and binds primarily to manglc saccharides present in cell surface glycans 17. To study this interaction the lectins were labeled with fitc and a fluorescence assay was performed. This paper reports the overall effects of three lectins, extracted from canavalia brasiliensis, dioclea violacea, and d.
Neutrophilinfiltrated paw edema induced by mannose. Jun 20, 2011 furthermore, dwl displayed affinity for simple sugars such as dglucose and dmannose at minimal inhibitory concentration of 62. Passiflora x violacea violet passion flowerrhs gardening. The data were submitted to the variance analysis, and the averages compared by the tukey test to 5% of probability and regression analysis. The first lectin was obtained from seeds of legumes from rio grande. Dieses kostenlose tool erlaubt es mehrere pdfs oder bilddateien miteinander zu einem pdfdokument zu verbinden. The lectins are from canavalia brasiliensis, canavalia bonariensis, cratylia floribunda, dioclea rostrata, dioclea virgata, dioclea violacea, and dioclea guianensis.
Quaternary structure of dioclea grandiflora lectin assessed. A lectin from dioclea violacea interacts with midgut. Thecanavalia brasiliensis, dioclea rostrata, anddioclea virgata lectins induced histamine release in rat peritoneal mast cells similar to concanavalin a. In the present study, we evaluated the effects of dioclea violacea lectin administered prior to ischemiareperfusion irinduced acute kidney injury aki. A study of the pharmacological actions of dioclea grandiflora. What type of interspecific interaction does dioclea megacarpa have with carydes from bio 171 at university of michigan. Text is available under the creative commons attributionsharealike license. Study on the vasorelaxant properties of dioclea lectins author links open overlay panel ito l. Dwl, a lectin extracted from the seeds of dioclea wilsonii, is a metalloprotein with strong agglutinating activity against rabbit and abo erythrocytes, inhibited by glucose and mannose. Quantification of the total phenolic and flavonoid contents, and antibacterial activity of dioclea reflexa edewor ti1, akpor ob2, owa so2 1department of pure and applied chemistry, ladoke akintola university of technology, ogbomoso, nigeria. Study on the vasorelaxant properties of dioclea lectins article pdf available in international journal of biological macromolecules 8215. Ipni nomenclature info for dioclea reflexa nomenclatural information about dioclea reflexa is provided by the international plant names index ipni. Dioclea violacea martius, ex bentham, contain a lectin that could be purified by affinity chromatography on sephadex g50 column.
Crystal structure of dioclea violacea lectin and a. Pdfdateien miteinander zu verbinden bezeichnet man auch als pdfmerge. Spatula restrains the growth of the developing arabidopsis seedling c w evemarie josse,a yinbo gan,b,1 jordi boutorrent,c kelly l. Pdf merge combinejoin pdf files online for free soda pdf. Dioclea grandiflora lectin thermodynamic analysis of the multivalency effect received for publication, october 21, 1999, and in revised form, january 19, 2000 tarun k. Resolution of the three dimensional structure of lectin. Dioclea e um genero botanico pertencente a familia fabaceae o wikispecies tem informacoes sobre. The acute treatment of mice with an ethanolic extract from the seed pod of dioclea grandiflora edgp at doses of 75, 150 and 300 mgkg by intraperitoneal administration produced a significant antinociceptive effect as displayed by the acetic acidinduced writhing test and the formalin test. Dsl appears to be composed of three polypeptide chains weighing approximately 30, 14 and 12 kda, matching the pattern of other diocleinae lectins, such as dioclea violacea and dioclea grandiflora. Dioclea state, a medieval serb state of duklja, in southeastern part of modern montenegro dioclea in praevalitana, an ancient roman and byzantine city in the province of praevalitana, near modern podgorica in montenegro. Dioclea altissima, dioclea rostrata 14, dioclea violacea 15, dioclea grandiflora 16, dioclea guianensis 17, dioclea sclerocarpa 18 and dioclea lasiocarpa 19.
An antinociceptive protein candidate to structurefunction analysis. You are using a web browser that we do not support. Mass spectrometry and xray diffraction analysis of two crystal types of dioclea virgata lectin. Crystallization and characterization of an inflammatory. This is a family of about 900 species, mainly of temperate regions, where they are usually small perennial plants of the viola type, and herbs, shrubs and trees from tropical regions whose flowers are significantly different from the familiar violet. Simple, entire or variously toothed, with a marked tendency to be cordate in form, all basal in some species, but in others alternate or rarely opposite hybanthus, stipulate, stipules permanent and foliaceous viola spp. The antinociception was observed through the first. Dioclea violacea lectin ameliorates oxidative stress and. These characteristics correspond to the pattern of other diocleinae lectins, such as dioclea altissima, dioclea rostrata, dioclea violacea, dioclea grandiflora, dioclea guianensis, dioclea sclerocarpa and dioclea lasiocarpa.
Pereirajunior, kyria santiago do nascimento, celso shiniti nagano, alexandre holanda sampaio, plinio delatorre, bruno anderson matias rocha, benildo sousa cavada, crystal structure of the lectin of camptosema pedicellatum. Unlike other plant lectins, both dgl and con a bind with high. This study investigated the effects of dioclea violacea lectin dvl on larval development in anagasta kuehniella. Original article flavonoid isolated from stem bark of dioclea. The plant list includes 95 scientific plant names of species rank for the genus dioclea. Purification, partial characterization and immobilization. What type of interspecific interaction does dioclea megacarpa.
Structural analysis of a dioclea sclerocarpa lectin. Neutrophilinfiltrated paw edema induced by mannosebinding dioclea violacea lectin author links open overlay panel nylane maria n. Crystal structure of the lectin of camptosema pedicellatum. The lectin from the seeds of dioclea grandiflora dgl is a manglc specific tetrameric protein with physical and saccharidebinding properties reported to be similar to that of the jack bean lectin concanavalin a cona. Dlyl showed no staining by periodic acidschiff, suggesting that it is not a glycoprotein.
Molecules 20, 18 10861 dlyl was able to maintain its hemagglutinating activity within a relatively wide ph range with. Therefore, a more detailed understanding of threedimensional structures of these proteins is essential for accurate analyzing the relationship between structure and function. Entomotoxic properties of dioclea violacea lectin and its. Pdf isolation and partial characterization of a lectin from seeds of. Unter dem namen hat sich ein kostenloses onlineangebot. Media in category dioclea violacea the following 21 files are in this category, out of 21 total. Discover lifes page about the biology, natural history, ecology, identification and distribution of dioclea multiflora boykins clusterpea discover life. An dioclea violacea in nahilalakip ha genus nga dioclea, ngan familia nga fabaceae.
Pdf mass spectrometry and xray diffraction analysis of. These lectins have presented high stimulatory capacity on lymph node t cells. All structured data from the file and property namespaces is available under the creative commons cc0 license. The histamine releasing properties of glucose mannosespecific lectins isolated from brazilian beans was examined. The integrated taxonomic information system itis provides authoritative taxonomic information on dioclea reflexa, as well as other plants, animals, fungi, and microbes of north america and the world. Molecular characterization and tandem mass spectrometry. The results showed that only dioclea violacea lectin dvl was able to interact with midgut glycans, unlike cratylia floribunda lectin cfl and canavalia gladiata lectin cgl. The dioclea violacea lectin was purified by affinity chromatography on a column of sephadex g50. Differences in structure of lectins were found to be reflected in differences in vasorelaxant effects based on their high specificity and selectivity for cell glycans.
Pagka karon wala pay siak nga nalista ubos niini niya. Dioclea state, a medieval serb state of duklja, in southeastern part of modern montenegro dioclea in praevalitana, an ancient roman and byzantine city in the province of praevalitana, near modern podgorica in montenegro dioclea in phrygia, an ancient city and former bishopric in phrygia asia minor dioclea plant. This free online tool allows to combine multiple pdf or image files into a single pdf document. Soda pdf merge tool allows you to combine two or more documents into a single pdf file for free. It was observed that the dormancy overcome of dioclea violacea seeds can be done with chemical scarification, 3 to 5 hours in. Therefore, the goal of the present study was to evaluate if treatment with dvl lectin. Daldinia eschscholtzii ascomycota, xylariaceae isolated from the brazilian amazon. Pdf isolation and partial characterization of a lectin. The seeds of these legumes are buoyant drift seeds, and are dispersed by rivers. The genus dioclea is in the family leguminosae in the major group angiosperms flowering plants. Viola primulifolia is very similar and closely related to v.
This simple webbased tool lets you merge pdf files in batches. The record derives from ildis data supplied on 20100714 which reports it as an accepted name record 16815 mirror. The record derives from ildis data supplied on 20100714 which reports it as an accepted name record 16815. Sdspage revealed three electrophoretic bands corresponding to the. Antinociceptive and toxicological effects of dioclea. In this study lectins purified from the seeds of dioclea violacea dvl and dioclea rostrata drl were compared with regard to crystal structure and vasorelaxant. Studies with dioclea violacea dvl, a lectin from the leguminosae family, diocleinae subtribe, showed that neutrophil migration was inhibited in different models of inflammation when applied intravenously 16,17, indicating that the lectin from dvl could be a promising substance for use in ameliorating the effects of aki. The sdspolyacrylamide gel electrophoresis patterns of the subunit structures of the lectins resemble cona and d. Data source and references for dioclea multiflora boykins clusterpea from the usda plants database. Data source and references for dioclea multiflora boykins. Files are available under licenses specified on their description page. Ipomoea violacea is a perennial species of ipomoea that occurs throughout the world with the exception of the european continent. Fred breweri from the departments of molecular pharmacology, and microbiology and immunology, albert. The protein was crystallized in the presence of the ligand xman and the crystals were obtained by the vapor diffusion method in hanging drop by sparse matrix.
The plant list includes a further 8 scientific plant names of infraspecific rank for the genus dioclea. Furthermore, when dvl was blocked with dmannose the interaction was inhibited. If you are looking for a way to combine two or more pdfs into a single file, try pdfchef for free. Usda, ars, germplasm resources information network. This work describes the evaluation of the antioxidant activity in the system bcarotene linoleic acid and radical scavenging effects of the substances 2,4dihydroxychalcone, 2,3,4,4tetrahydroxychalcone butein, 4methoxy2,3,4trihydroxychalcone, 7hydroxyflavanone, 3,4,7trihydroxyflavanone butin, 7,4dihydroxyflavanone liquiritigenin, 6methoxy7. In this study lectins purified from the seeds of dioclea violacea dvl and dioclea rostrata drl were compared with regard to crystal structure and vasorelaxant properties. Entomotoxic plant lectins have been extensively studied in the past two decades, yet the exact mechanisms underlying their toxic effects remain unknown. We used four lectins extracted from legumes, such as dviol, drl, conbr and lsl, respectively extracted from dioclea violacea, d. Lectin from dioclea violacea induces autophagy in u87. Genus passiflora are mostly tender tendrilclimbing shrubs, with simple or palmatelylobed, generally evergreen leaves and showy flowers of distinctive structure with often colourful coronal filaments, followed by conspicuous fruits, edible in some species. Laboratory of evaluation of drugs psychobioactive and toxicology. The dioclea violacea lectin dviol was purified on a sephadex g50 column and crystallized in the presence of the xmannose by the vapor diffusion method. Chronic exposure of larvae from neonates to the fourth instar demonstrated that dvl interfered with larval growth, retarding. Dioclea is a genus of flowering plants in the pea family, fabaceae, that is native to the americas.
The seed lectin of dioclea sclerocarpa dsl, similar to other diocleinae lectins, is a glucosemannose ligand protein that has a monomer with a molecular mass of 25,606 da, associated as tetramer. Jul 27, 2015 the structural basis of the ph dependency of the dimertetramer transition exhibited by brindas type ii diocleinae lectins was investigated by equilibrium sedimentation and x. In this study lectins purified from the seeds of dioclea violacea dvl and dioclea rostrata drl were. Daldinia eschscholtzii ascomycota, xylariaceae isolated from. Molecular characterization and tandem mass spectrometry of. Access is allocated within two business days of purchase and lasts one year. This variability lies in small differences that can be analyzed in studies based on structures. Therefore, the goal of the present study was to evaluate if treatment with dvl lectin could improve the renal function of rats with aki induced by renal ir. Neutrophilinfiltrated paw edema induced by mannosebinding dioclea violacea lectin nylane maria n. Manual adjustments in coot were made after each refinement step and an xman molecule was finally added. Dioclea este artigo sobre leguminosas familia fabaceae, integrado no projeto plantas e um esboco.
Diocleinae lectins are a group of proteins with conserved. Jun 18, 2014 this page was last edited on 3 september 2017, at 07. This page was last edited on 14 december 2019, at 15. Isolation and characterization of novel lectins from. Pdf structural analysis of a dioclea sclerocarpa lectin. If you have any comments about the site or the pictures, good or bad, wed like to hear from you. This page was last edited on 3 september 2017, at 07. Harder1, and przemyslaw prusinkiewicz2 1department of biological sciences, university of calgary, calgary, alberta, canada t2n 1n4 and 2department of computer science, university of calgary, calgary, alberta, canada t2n 1n4. Our pdf merger allows you to quickly combine multiple pdf files into one single pdf document, in just a few clicks. Ang dioclea violacea sakop sa kahenera nga dioclea sa kabanay nga fabaceae.
Pdf zusammenfugen online pdf dateien zusammenfugen. In particular, dioclea sclerocarpa seed lectin dsl presents low ability to relax endothelialized rat aorta in comparison with other dioclea lectins such as dioclea violacea dvl, dioclea virgata dvirl and dioclea. Please note that this is a trial program and may end. A nutritional study of dioclea grandiflora mucuna and dioclea sclerocarpa, two legume seeds related to canavaliawhich grow extensively in south america and are used as a human food source, has. Dioclea or diocleia, and also dioklea or diokleia may refer to. Reports mention sedative, tonic, and also antiepileptic properties for this plant. Crystallization kits were used to obtain protein crystals and the. It is most commonly called beach moonflower or sea moonflower as the flowers open at night.
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